Chroma Technology Corporation® Precision Optical Filters and Coatings

ET Series Sets for Fluorescence Microscopy

High-throughput Modified Magnetron sputter-coated filter sets provide high throughput in many applications, and more durable coatings for adverse environmental conditions.

Typical transmission through excitation and emission filters has been increased to 93–97%. This, combined with stricter spectral tolerances and the pass-band steepness of the HQ series filters, results in significantly improved signal detection when protein expression or dye labeling is uncertain. The brightness of a typical fluorochrome can often be increased over 50%, or the exposure time can be reduced roughly in half.

Many of the new filters will be made on a single substrate, allowing more flexibility in design. The new filters are directly coated with durable materials on a custom glass or fused-silica substrate. The ET filters do not rely on epoxy for environmental durability and none of the current ET sets have epoxy in the light path. Though the coatings are exposed, they are remarkably tough.

Not all the individual filters in the ET series will use Modified Magnetron technology. Since too much UV excitation light can be deleterious in many applications, the new DAPI set will use an attenuated excitation filter matched with a high-throughput ET emission filter.

Further information on ET Series sets is available from Chroma Technology Corporation.

GFP Sets

This catalog in PDF Format describes most of the Chroma Technology Corporation filter sets for viewing and imaging the various GFP mutants. For each filter set it provides a spectral trace and, when applicable, a note on the unique quality of that set.

Other Sets

If the set you need is not mentioned above, please search for a Filter Set on Chroma Technology Corporation’s Web site.

Further Information

Please contact BioVision Technologies if the filter set lists do not contain the combination that you require.