Deconvolution Software

Huygens® Essential Image Processing Software

Huygens® Essential is an image processing software package tailored for deconvolution of microscopic images. Its wizard‐driven user interface guides you through the process of deconvolving microscopic images. Huygens Essential is able to deconvolve a wide variety of images ranging from 2D widefield (WF) images to 4D multi-channel two-photon confocal images. To facilitate comparison of raw and deconvolved data, or comparison of results from different deconvolution runs, Huygens Essential is equipped with a dual 4D slicer tool. Powerful and fast renderers produce different representations of your data, not needing any special graphic card.

Key Features

• Wizard‐driven interface;
• Five‐stage guided deconvolution procedure;
• Extensive image parameter checking;
• Creates histogram to spot problems due to saturation early on;
• Automatic estimation of the background;
• Automatic bleaching correction;
• Automatic deconvolution of time series;
• Automatic correction for spherical aberration;
• Guides you along multi‐channel deconvolution;
• Comparison viewer enables you to inspect and compare images;
• Z-drift correction;
• Thumbnail representation of images in progress;
• Standard up to 32 channels;
• You can measure, store and deconvolve using your own point spread function (PSF);
• Reads ICS, ICS2, OME, Imaris classic, numbered name TIFF series, Zeiss LSM, Multi-layer TIFF, MetaMorph STK, Biorad PIC, Olympus ‘Fluoview’ TIFF, Olympus SIS Tiff series, Olympus OIF, Leica LIF files, Leica numbered name TIFF series, Delta Vision IMSubs (r3d), IPLab TIFF, IPLab IPL, iVision‐Mac IPM.
• Writes ICS, ICS2, OME, Imaris classic, Biorad PIC, Delta Vision IMSubs (r3d), Single‐layer TIFF, Multi‐layer TIFF, Leica numbered name TIFF series.
• Comes with one full year of maintenance.
• Remote display
  • via Remote Desktop Protocol if running on Microsoft Windows operating system;
  • via X11 protocols if running on Mac OS X or Linux;
• Automatic and manual cropping of datasets in five dimensions (3D + time + channels);
• Twin Slicer: easy comparison of original and deconvolved data, or of different slices in your data;
• Volume renderer (“SFP Renderer”) enables you to inspect and compare images in 2D, 3D and 3D time;
• Fast Surface Renderer, to easily explore the different objects present in your data via isosurface rendering or maximum intensity projection;
• 32‐bit version available for:
  • Pentium III‐class computers or better running Linux;
  • 32‐bit editions of the Microsoft Windows operating system;
  • PowerPC or Intel Mac OS X 10.4 “Tiger” and 10.5 “Leopard.”
• 64‐bit version available for:
  • X86‐64 computers running Linux;
  • 64‐bit editions of the Microsoft Windows operating system;
  • PowerPC or Intel Mac OS X 10.5 “Leopard.”

Demonstration software is available from Scientific Volume Imaging’s Web site.

Deconvolution references are available.