Roper Scientific iLas2 from BioVision Technologies
Dual Laser Illuminator for TIRF Microscopy and Simultaneous Targeted Laser Action
The iLas2 system is a unique multi-application device that offers complete control over any other laser illumination. It provides researchers the ability to manage and modify the position and focalization of laser light in real time.
It supports TIRF microscopy, FRAP, uniform wide‐field laser illumination with limited background signal, photoactivation, and photoablation.
It works closely with MetaMorph acquisition software, configuring the Multi Dimensional Acquisition dialog to acquire images according to the protocol established within iLas2.
iLas2 setting up Multi Dimensional Acquisition for TIRF
TIRF
Total Internal Reflection fluorescence (TIRF) microscopy is particularly useful for observations close the coverslip surface as it provides the highest axial resolution available. This technique covers a large field of applications, such as single molecule tracking, interaction at the cell membrane level or actin filament behavior.
FRAP
The iLas2 system is a solution designed to perturb the intracellular dynamics of proteins and other macromolecular complexes via FRAP (fluorescence recovery after photobleaching), photo‐conversion, un‐caging, and photo‐ablation experiments. It is a modular system which includes a galvanometer‐based optical head, the associated electronics and a software pack powered by MetaMorph acquisition software. It uses illumination paths of conventional research microscopes and is compatible with 3‐D widefield microscopy, a spinning disk confocal system, and Total Internal Reflection Fluorescence microscopy. Combined with a 3‐D sectioning devices, 3‐D acquisitions and photo-perturbation are possible.
2‐D FRAP, 4‐D FRAP, and 4‐D Photoactivation
Hardware Specifications
- Stand alone TIRF/FRAP/WF system
- Fastest motorized TIRF angle <1ms response time
- Perfect TIRF/widefield illumination uniformity
- 20,000 laser positions a second in vectorial control mode
- Diffraction limited laser spot
- Superimpose all optical paths
- No computation delays and positioning issues
- 350-650nm light range
Software
- Stand‐alone software embedded within MetaMorph software
- Operates with MetaMorph, configuring Multi Dimensional Acquisition experiments
- Independent laser control
- Independent adjustment of penetration depths
- Fast motorization of TIRF angle
- Live switching between TIRF, FRAP, WF