iVision‐Mac™: Software Features

Capture images using popular scientific grade CCD cameras.

Capture images at specified time intervals. Measure and plot intensities over time.

Control motorized microscope stands from popular manufacturers.

Control third-party devices: shutters, filter wheels, stages.

Capture through-focus series using Z-axis focus drives.

Capture images from multiple wavelengths, overlay colors, and quantify intensities.

Create a list of files to process all at once. Indexed files provide automatic file naming and numbering.

Split any 24- or 48-bit color image into the separate components. View the separated images in their corresponding colors. Operate on the color component images as you would any grayscale image.

Build a 24- or 48-bit color image out of three separate image files or sequences. Blend a fluorescent image or sequence with a DIC or other grayscale image.

Combine and compare two images on a pixel-by-pixel basis. Perform background subtraction and image averaging to reduce noise. Mask certain frequencies in the Fourier domain.

Choose from an extensive list of built-in mathematical operations to apply to each pixel for analysis and enhancement.

Arbitrary rotation, scale and translation with sub-pixel accuracy. Choose nearest neighbor or bilinear interpolation methods. Also transpose and flip images to view them differently.

Complex multiplication of Fourier spectrum real and imaginary components. Convert between real-imaginary and magnitude-phase.

Forward and inverse 1-D and 2-D Fast Fourier transform with results in either real-imaginary or magnitude-phase. Forward and inverse cosine transform. All transformations are done with floating-point values for best accuracy.

Exchange image data with other programs through cut-and-paste.

Enhance brightness and contrast, and apply a gamma curve to the displayed image data.

Easily perform flat-fielding to correct for lighting and sensor non-uniformities.

Choose from built-in sharpening, smoothing, and median filters; pseudocolor tables, contrast enhancements and tools for geometric re-sizing.

Define your own linear filter kernels.

Add registration marks to several images and let iVision‒Mac automatically rotate, scale and shift the images to bring them into alignment.

Rotate, Scale, Flip.

Easily transfer information from one image to another, including the ROI and object definitions, color table, overlays and units.

Complete control over the color lookup table to pseudo-color images, equalize histograms and stretch the image contrast.

iVision‒Mac has a long list of measurements you can perform, for measuring density, shape, position and intensity moments. Limit measurements to specific regions and/or value ranges.

Count objects and measure density, shape, position, and intensity moments for individual objects and for groups. Limit measurements to specific regions and/or value ranges. Automatically label measured items. Export results to spreadsheets.

Integrated and mean densities, RMS and standard deviation, max and min values.

Plot image data using color histograms. Customizable or automatic binning.

Record your own macros to automate your experiments. Run reproducible protocols as if they were a single command. Assign your most common scripts to keyboard function keys. Turn complex procedures into a sequence of labeled key-presses. There is no scripting language to learn; just point and click.

Compute optical density, perform accurate ratios of images taken at different wavelengths.

Our integrated graphing functions produce publication-quality graphs of your data and analysis results.

Calibrate measurements in units of your choice – microns, mm, cm, inches, etc.

Plot data values along a slice through the image, or list the (x,y) coordinates and data values at each point along the boundary of an object.

Click the mouse to quickly measure lengths and angles. Automatically creates table of measurements and marks the measured lines and angles on the image within a nondestructive overlay.

Interactively select thresholds to separate objects from their background.

Erosion, dilation, opening and closing filter operations, with user-definable kernels.

Plot the average value or the sum of all the pixels along a row or column of a rectangular or polygonal region.

Customize iVision‒Mac further by programming your own algorithms. The manual gives complete instructions and examples for including your own code and dialogs written using CodeWarrior for Macintosh and Microsoft Visual C++^TM.

Add or replace values to the region of interest: horizontal and vertical ramps, Gaussian and uniform random noise.

Non-destructive overlays let you draw text and graphics on top of your images without altering the underlying data values. iVision‒Mac saves both the overlay and image data.

Read and write files in a number of formats: TIFF, Text, and our own iVision‒Mac format. iVision‒Mac also handles IPLab/Windows IPLs, PICTs, FITS, and EPR (*.raw and *.psf) formats. iVision‒Mac’s powerful Open As command imports foreign files in almost any file format.

Create a movie loop from your time lapse and 3D images. Play it in iVision‒Mac or export it as a QuickTime movie files.

Switch easily between a text view and a standard image view of your data.

Easily export results to Microsoft® Excel® spreadsheet software. Save your data as text and easily open it in Excel® or any other spreadsheet program.

Capture multiple images and place them together in one large montage.

Print images as halftones to any PostScript printer or QuickDraw compatible printer. Make a PostScript file of your image data.